PPARA Stable Expression Cell Line (HCT 116)

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  • 细胞图0 PPARA Stable Expression Cell Line (HCT 116)-细胞图0
  • 细胞图1 PPARA Stable Expression Cell Line (HCT 116)-细胞图1
  • 质粒图0 PPARA Stable Expression Cell Line (HCT 116)-质粒图0
  • PPARA Stable Expression Cell Line (HCT 116) PPARA Stable Expression Cell Line (HCT 116)-产品图
细胞图0 PPARA Stable Expression Cell Line (HCT 116)-细胞图0
细胞图1 PPARA Stable Expression Cell Line (HCT 116)-细胞图1
质粒图0 PPARA Stable Expression Cell Line (HCT 116)-质粒图0
PPARA Stable Expression Cell Line (HCT 116) PPARA Stable Expression Cell Line (HCT 116)-产品图
PPARA Stable Expression Cell Line (HCT 116) Copy Product Info
Catalog#： YOE-L051
Size： 1*10^6
Culture method： 90%McCOY's 5A+10% FBS
STR Report
Datasheet (Use Instruction)
  • 细胞图0 PPARA Stable Expression Cell Line (HCT 116)-细胞图0
  • 细胞图1 PPARA Stable Expression Cell Line (HCT 116)-细胞图1
  • 质粒图0 PPARA Stable Expression Cell Line (HCT 116)-质粒图0
  • PPARA Stable Expression Cell Line (HCT 116) PPARA Stable Expression Cell Line (HCT 116)-产品图
PPARA Stable Expression Cell Line (HCT 116) Copy Product Info
Catalog#： YOE-L051
Size： 1*10^6
Culture method： 90%McCOY's 5A+10% FBS
Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Catalog YOE-L051 Transcript NM_001001928.4
Cell name PPARA Stable Expression Cell Line (HCT 116) Species Human
Gene Name PPARA Passage ratio 1:2~1:4
Cell Morphology Epithelial-like, adherent Antibiotic Concentration Puro=0.5μg/ml
Culture method 90%McCOY's 5A+10% FBS Mycoplasma test negative
Catalog
YOE-L051
Cell name
PPARA Stable Expression Cell Line (HCT 116)
Gene Name
PPARA
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Transcript
NM_001001928.4
Species
Human
Antibiotic Concentration
Puro=0.5μg/ml
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS

Product Description

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined.
Gene Expression Validation
No. Sample hPPARA-1CтMean β-h_actinCтMean ΔCтMean ΔΔCт Expression Rate
1 PPARA Stable Expression Cell Line (HCT 116) 18.877 16.548 2.329 -6.683 102.750
2 HCT 116_Ctrl(Puro) 25.415 16.403 9.012 0.000 1.000
STR Profile
Download STR Report
Loci STR Info (Sample Cell)
Sample Cell Line:HCT 116		 STR Info (Cell bank)
Cell Line:HCT 116
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 10121012
D7S820 11121112
D16S539 11131113
VWA 17221717
TH01 8989
AMEL XXXY
TPOX 8889
CSF1PO 710710
D12S391 1721
FGA 1823
D2S1338 1616
D21S11 2930
D18S51 1617
D8S1179 1214
D3S1358 1219
D6S1043 1313
PENTAE 1314
D19S433 1213
PENTAD 913
D1S1656 1214
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    High expression
    Exclusive cell selection technique, ensuing high expression, RT-qPCR validation data provided.
  • icon
    Good stability
    Stable gene copy number, low passage, can be stably passaged for more than 20 passages.
  • icon
    Strict QC
    Strict quality control, ensuing no bacterial and mycoplasma contamination, STR authentication and cell post-thawing viability test passed; High standard delivery.
Citations:
Product Catalog#：YOE-L051
Product Name：PPARA Stable Expression Cell Line (HCT 116)
Related Resources: Red Cotton Gene-editing System | ATCC | DSMZ | ECAZZ | Cellosaurus | CCTCC | NCBI
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